bsai digested sgrna vector (Addgene inc)
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Addgene inc
bsai digested sgrna vector
Bsai Digested Sgrna Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 168 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bsai+digested+pgl3+u6+sgrna+pgk+puromycin+vector/pGL3-U6-sgRNA-PGK-puromycin+(Plasmid+%2351133)/pm41484129-482-8-11
Average 94 stars, based on 168 article reviews
Bsai Digested Sgrna Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 168 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bsai+digested+pgl3+u6+sgrna+pgk+puromycin+vector/pGL3-U6-sgRNA-PGK-puromycin+(Plasmid+%2351133)/pm41484129-482-8-11
Average 94 stars, based on 168 article reviews
bsai digested sgrna vector - by Bioz Stars,
2026-10
94/100 stars
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Sequencing:Article Title: Generation and characterization of stable pig pregastrulation epiblast stem cell lines. Article Snippet: Second, to obtain NANOG-tdTomato knock-in cell lines, we constructed a NANOG DNA donor vector with four fragments, Backbone, Left Homology Arm-3× Flag, 3× Flag-P2A-tdTomato-Loxp-PuroLoxp and Right Homology Arm, using NEBuilder® HiFi DNA Assembly Master Mix (NEB, E2621X) as previously described.89 The NANOG sgRNA targeted the sequence before the stop codon site to knock in the donor fragment as a reporter. .. The annealed sgRNA sequence was cloned into the Clone Assay:Article Title: Generation and characterization of stable pig pregastrulation epiblast stem cell lines. Article Snippet: Second, to obtain NANOG-tdTomato knock-in cell lines, we constructed a NANOG DNA donor vector with four fragments, Backbone, Left Homology Arm-3× Flag, 3× Flag-P2A-tdTomato-Loxp-PuroLoxp and Right Homology Arm, using NEBuilder® HiFi DNA Assembly Master Mix (NEB, E2621X) as previously described.89 The NANOG sgRNA targeted the sequence before the stop codon site to knock in the donor fragment as a reporter. .. The annealed sgRNA sequence was cloned into the Plasmid Preparation:Article Title: Generation and characterization of stable pig pregastrulation epiblast stem cell lines. Article Snippet: Second, to obtain NANOG-tdTomato knock-in cell lines, we constructed a NANOG DNA donor vector with four fragments, Backbone, Left Homology Arm-3× Flag, 3× Flag-P2A-tdTomato-Loxp-PuroLoxp and Right Homology Arm, using NEBuilder® HiFi DNA Assembly Master Mix (NEB, E2621X) as previously described.89 The NANOG sgRNA targeted the sequence before the stop codon site to knock in the donor fragment as a reporter. .. The annealed sgRNA sequence was cloned into the |